NMMU's Madibaz will play against the Tshwane University of Technology (TUT) in the Varsity Football Challenge semifinal in Pretoria tonight (7pm).
The teams shared the spoils when they met in the round-robin stage, but this time the Madibaz are chasing victory.
Both sides are unbeaten so far.
The Madibaz qualified for the knockout stages after finishing fourth on the log table, while TUT finished top.
Second-placed University of Pretoria and North West University will meet in the other semifinal, also today.
Madibaz team manager Mark Tommy said his team's clash was bound to produce fireworks.
"We gave them a tough time in our last encounter when we scored a late equaliser in the dying minutes but this time we want to get an early first goal and settle down," Tommy said.
"Our last game with TUT was regarded as the best game of the tournament and we will approach this coming encounter as a final because TUT are a quality team."
Tommy said the fact that the Madibaz had reached the semifinals proved they could compete against any other varsity football team.
"We will be playing to our strengths and we will be out there to match the TUT in all areas of the game.
"TUT's weakness is that their players and supporters are arrogant and they believe they can beat any varsity team in the country, but we proved them wrong last time we clashed, when we drew 2-2."
The Madibaz will be boosted by the return from suspension of quality defender Nevin Kopps and super striker Leroy van Rensburg, who was injured.
"We have a full-strength squad for this game and we will rely on our strikers up front to convert those goals because this is the most important game of the tournament so far. This is a final before the final."
TUT manager Archie Mogale said the Madibaz were up for a tough challenge against his side.
"We never expected we would play against them in the semifinals because we thought we would meet them in the final," Mogale said.
"They are very good and we will respect [them] but they must expect a completely different team for this encounter, better than the first one when we drew against them in PE."
Mogale said they would not underestimate the Port Elizabeth outfit because they stood a good chance of winning the tournament.
The match will be televised live on SuperSport Select from 6.45pm.